The tight integration of the required light engines (femtosecond laser, 8-channel cw light engine, white light source) together with the corresponding detection modules and beam handling optics allows an entirely new way of implementing and using a multi modal microscope. The microscope becomes a truly turn-key, integrated, compact, easy-to-use, maintenance-free and very easy-to-install imaging platform.
The user does not need to worry about laser alignment, detection efficiency or non-compatible or misaligned hardware over time. The entire microscope consists of two parts, controlled through a flexible, non-detachable umbilical, the scanhead and the controller box. The compact scanhead incorporates laser beam handling and scanning, detectors and control electronics. Everything is pre-aligned, rigid and firmly fixed. The scanhead can be rotated and translated in any direction (truly 3D) and enables all multi-photon-based imaging needs. Cells, molecules, even live whole animal imaging can be accomplished.
The controller on wheels incorporates a built-in PC together with other electronic and photonic devices. Only a power cord, keyboard, mouse and display need to be connected to the controller box to make it completely operational. The device can easily be operated even by non-experts. It allows, through the built-in multimodality, extra features and combination of imaging capabilities for correlative microscopy which is not available until now. It is simple to use, and has the utility of a household appliance. Researchers can share the microscope; it is easily moveable and transportable between different locations. This novel capability is unique to the field, and serving a growing need. A modular and flexible product layout allows the user to upgrade the microscope at a later stage and ensures the researcher can add extra features over time on the same platform.
Currently, you have the possibility to scan one sample in single-photon, two-photon and brightfield modality.
We are planning for a FLIM application, and a coherent RAMAN source, as well as a non-coherent one. We are also working on a THG modality at the moment.
The user can freely choose between the built-in modalities (1P, 2P and brightfield) to image their samples with a broad spectrum of corresponding modalities. The options are limitless. They can then easily overlay/correlate images and process the multidimensional/multimodal data with our powerful software.
The laser wavelength is fixed at around 1030nm for now. SHG and 3PEF works, for THG the laser wavelength is still a little bit too short, as THG will be generated too far in the UV range (although it will work in forward direction).
Yes, one can use up to 4 PMT channels simultaneously. We typically use Semrock filters (long-pass dichroic and notch filters).
100mW is indeed on the low side, however with 1000mW in galvo-galvo configuration you will burn the samples.
Yes, we are using ScanImage as scan software. The lowest resolution mode is 64x64.
Yes, there is the possibility to add a resonant scanner to the basic version.
We have tested up to 0.8NA (Nikon 16x). The higher the NA, the better!
Our software enables the chose, independent of the modality, of any of the 4 positions on the turret. The Customer can mount any objective on any position and choose it independently.
As the scanhead is freely movable (translation & rotation) in all directions, one can move along any axis. However, we don´t have a script that does that automatically. We can deliver such software on demand.
There will be no re-alignment necessary. Our microscope will arrive turn-key out of the box.
These modalities are optional.
No, not yet.
Yes. Typically, we use ImageJ for that.
What range? We need to check that.
No limit basically (the umbilical is 2m long).
Yes. Please see our application section for examples.
We are improving the scanning speed every day. Currently it takes approximately 1 hour to achieve this area.
Ideally it should be flat. If we get 3-dimensional tissues samples, we gently press them against the glass bottom, of the Fluorodish (or any other) plate.
Yes, we do. There is the possibility to sign a service contract. This contract contains: phone support, repair of broken devices and replacement devices. Please contact us for further information.
Depends on the galvo-scanangle: You can choose between 60 µm and up to 1200 µm
For 2P-Microscopy you can image samples with a thickness of up to 200 µm . Compared to a conventional confocal Fluorescence microscope (50-80 µm), the penetration depth for NIR laser light is higher, because of less light scattering.
This makes the 2P-Microscopy a suitable technique to image tissue and 3D cell culture samples.
Yes, you can use standard fluorescence dyes and use filter-sets optimized for your spectral range. In addition, you can optimize your experiments by using special commercially available 2P high-absorbance fluorescence dyes.
You can save the images as tiff-files and pre-processing the RGB-images (1PM) or 16-bit grayscale (2PM) images with the image editing software of your choice.
No, we are working with well-established software like e.g. Windows10, ScanImage, µManager, Fiji or MATLAB. All software will be pre-installed and ready-to-use.
MATLAB-based, ImageJ and home-built GUI
Digital staining means taking advantage of autofluorescence and endogenous structures of your sample, to image different tissue and cell architecture and structures, metabolic conditions, pathogenic tissue and so on without the use of external staining’s.
The big advantages of digital staining are time-saving imaging, no manipulation of your sample by chemicals and dyes and no mis-bonding by dyes or antibodies and therefore no artefacts caused by staining procedures.