IMAGING SERVICE

Come by to visit or send us your sample!

There are many benefits in taking advantage of this service: you can come by, visit our facility and scan the samples together with our scientists. We are flexible. Another option would be to send us your samples for characterization. On request our scientist will video chat with you through the working process. We will scan the samples according to your specifications. You can be assured our scientist will handle your samples with premium care and caution.

The measurement equipment is checked, updated, and calibrated regularly to ensure the best quality and reliability possible for your scans.

Single-photon fluorescence setup:

  • Upright and inverted configuration
  • Illumination source: 8 discrete output bands through UV-NIR (380 – 750 nm), >200 mW / channel at the sample
  • Variable filter sets for experiment dependent customized imaging
  • sCMOS monochrome camera with >95% QE
  • Color camera for brightfield imaging

Multi-photon fluorescence setup:

  • Upright and inverted configuration
  • Laser light sources used in MPX-1040, MPX-920F and MPX-Tune: >600 mW <130fs 80MHz pulses at 1040nm; >200 mW <130fs 80MHz pulses at 920 nm
  • 4 simultaneous PMT channels for 2-photon, 3-photon, SHG and THG imaging, GaAsP PMTs with low dark current, special selection
  • Variable filter sets for experiment dependent customized imaging
  • Galvo-galvo scanning, up to 900 μm FOV
  • Pixel up to 2048 x 2048

MesoSPIM V5 light sheet:

  • Excitation laser with wavelengths: 405 nm, 488 nm, 638 nm
  • Sample positioning with motorized XYZ - Stage
  • Motorized zoom and focus module
  • Imaging with Teledyne Photometrics Prime BSI Express
    + > 95% QE
    + 2048 x 2048 pixels
    + low read noise of 1.8 e-
  • Part of the mesoSPIM community: https://mesospim.org

Whole slide imaging (WSI) setup:

  • Upright or inverted configuration
  • Automatic slide scanning
  • Fully automated slide loading (up to 200 slides)
  • Single-photon or multi-photon setup

Additional equipment:

  • Motorized stage for tiling and z-stack imaging
  • Piezo z-stage for high precision z-stacking.
  • Objectives: 16x Water (NA 0.8, WD 3.0), 20x Air (NA 0.75, WD 1.0), 25x Water (NA 1.0, WD 4.0), 40x Air (NA 0.95, WD 0.2), 60x Oil (NA 1.4, WD 0.21), 100x Oil (NA 1.4, WD 0.17), 4x Air (NA 0.2; WD 20.0)

IMAGING SERVICE

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